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After washing with PBS, the placental tissues were incubated with secondary antibodies for 1 h at 37C and developed with diaminobenzidine tetrahydrochloride, and the signal was measured using an inverted fluorescence microscope (Olympus, BX53F, Japan)

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10.1111/jphp.13095 35 WangD.WangX.GuX.ZhangY.JiangY.LiuY.et al (2024)

Microenvironment in Subchondral Bone: Predominant Regulator for the Treatment of Osteoarthritis

Because superoxide is ubiquitous, different SOD isoforms exist for specific cellular compartments, each requiring specific metal cofactors: Cytosolic SOD (SOD1): Requires copper (Cu) and zinc (Zn)