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To test whether it was feasible to divert the Cys precursors (i.e., Hcy and cystathionine) accumulated in the evolved strain to Cys synthesis and then increase GSH production, the two key genes encoding cystathionine -lyase (-CTLase, CYS3 ) and cystathionine -synthase (-CTSase, CYS4 ) of the Cys biosynthesis pathway were independently or simultaneously overexpressed in the evolved strain under the control of a constitutive promoter (Supplementary Fig

2015;2015:CD007259

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