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In brief, 30 ml of the HEK293 cell suspension culture in a 50 ml centrifuge tube (Violamo, #VIO-50BN) was centrifuged sequentially at 1000 g for 10 min and 3000 g for 10 min at 4 C to remove cell bodies and dead cells, respectively, using MX-307 centrifuge and AR510-04 rotor (TOMY, k-factor: 880)
The peptide also appears to have some direct effects on peripheral tissues beyond the central nervous system, including potential metabolic effects, cardiovascular influences, and immunomodulatory properties

The brain of each mouse was excised and kept at -80 C for estimation of reduced glutathione (GSH), lipid peroxidation (MDA) and nitric oxide determination in brain homogenate

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Much of the evidence comes from mechanistic studies, case reports, and retrospective analyses
