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The cDNA fragments were then blunt-ended through an end-repair reaction, followed by dA-tailing
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Cells were scrapped immediately after with 1.4 mL of a cold (0 C) CH 3 OH:CH 3 CN (50:50 v/v) solution and subsequently frozen using liquid N 2
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Rotate between at least 3 to 4 sites to prevent lipodystrophy and scar tissue buildup that can slow absorption over time
