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Of interest, the recovery of transferrin expression in Sertoli cells observed in our study suggests a role of IGF-I in maintaining the integrity of the hematotesticular barrier [23] and consequently on cellular proliferation in some tubuli

To avoid this issue, 4-fluorophenylhydrazine was added into the reaction mixture to quantitatively convert pyruvate to 2-(2-(4-fluorophenyl)hydrazono)propanoate ( 23 ) and then used for quantification using 1 H-NMR method (Supplementary Fig