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After washing with cell lysis buffer, bound protein was eluted with 20 mM HEPES pH 7.0, 500 mM NaCl, and 250 mM imidazole
Of the 14 semaglutide-treated patients that developed anti-semaglutide antibodies, 7 patients (0.2% of the overall population) developed antibodies cross-reacting with native GLP-1
