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Same protocol with one less reconstitution

Additionally, our study cohorts were vaccine-nave, while subjects in the other study had received 2 doses of SARS-CoV-2 vaccines

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J Clin Invest (2002) 109(3):36371

Option 1: Discard and mix fresh Cost: Price of new vial Benefit: Guaranteed full potency Risk: None (besides cost) Option 2: Continue using Cost: None (already paid for) Benefit: Don't waste remaining peptide Risk: 10-15% potency loss means 10-15% less effectiveness Calculation: If vial costs $50 and you're at day 35 with half remaining, you're deciding whether to waste $25 to guarantee full potency, or accept 10-15% reduced effectiveness