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Increased N-GSDMD was translocated into lipids on the plasma membrane, and further promoted cell lysis by oligomerizing and forming pores [120, 121]

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Capillary electrophoresis time-of-flight mass spectrometry measurement Fifty microliters of plasma were added to 200 L of methanol containing internal standards (Solution ID: H3304-1002, Human Metabolome Technologies [HMT], Tsuruoka, Japan) at 0 C to inactivate enzymes