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Furthermore, while GABAAR currents are increased by barbiturates (and other general anesthetics), barbiturates, as relatively non-selective compounds, bind to an entire superfamily of ligand-gated ion channels and can block ligand-gated ion channels predominantly permeable for cationic ions, the neuronal nicotinic acetylcholine receptor (nAChR), the 5-HT3 receptor, the glycine receptor, and others [231,232]

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Redox mobility shift assays For the redox mobility shift assays, proteins were purified, reduced and oxidized as described above

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Are physiologically immature and may handle peptide exposure differently than adults