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Human peripheral blood mononuclear cells PBMC from healthy donors, hyperthyroid GD patients and euthyroid GD patients were isolated from heparinized peripheral blood by density gradient centrifugation on Ficoll-Hypaque (Sigma Aldrich)

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Under hyperglycemic conditions, renal DCs mature through TLR4/NF-B signaling, upregulating co-stimulatory molecules (CD80/86) and secreting pro-inflammatory cytokines, thereby amplifying local inflammation (73)
