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For Gpr124 KO mice, both WT and heterozygous littermate embryos were used as control

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(6)Fmoc-Asp(otBu)-Asp(otBu)-Ala-Gly-Leu-Val-WangResin (6) Preparation of Fmoc-Asp(otBu)-Asp(otBu)-Ala-Gly-Leu-Val-WangResin (5)Fmoc-Asp(otBu)-Ala-Gly-Leu-Val-WangResin(20DMF)5203DMF3DMF2Fmoc-Asp(otBu)-OH1.1gDIEA0.4mlTBTU0.56gHOBT0.2gDMF10ml1.53DMF3DMF2Fmoc-Asp(otBu)-Asp(otBu)-Ala-Gly-Leu-Val-WangResin In the Fmoc-Asp(otBu)-Ala-Gly-Leu-Val-WangResin in step (5), add decapping reagent (20% piperidine in DMF solution), react at room temperature for 5 minutes, drain, and add decapping reagent at normal temperature React for 20 minutes, wash with isopropanol for 3 times, DMF for 3 times, DMF for redistilled for 2 times, drain, add Fmoc-Asp(otBu)-OH1.1g, DIEA0.4ml, TBTU0.56g, HOBT0 .2g, DMF10ml normal temperature reaction for 1.5 hours, drained with a vacuum pump, washed 3 times with isopropanol, washed 3 times with DMF, washed 2 times with DMF evaporated, and dried to obtain Fmoc-Asp(otBu)-Asp(otBu )-Ala-Gly-Leu-Val-WangResin