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Then the cells were collected on multiscreen GV filters under a vacuum and were subsequently washed twice with ice-cold 0.4N perchloric acid (70%) and 100% ethanol

These extensive metabolic modifications are primarily triggered by oncogenic signaling pathways and modified metabolic enzymes, enabling cancer cells to meet their growth requirements in environments with variable nutrient availability

10.1371/journal.pone.0253106 PLoS One 66 GangolaS.SharmaA.BhattP.KhatiP.ChaudharyP

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[DOI] [PMC free article] [PubMed] [Google Scholar] 14.Michaud K, Solomon DA, Oermann E, Kim JS, Zhong WZ, Prados MD
