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After freeze-thaw processing, supernatants were collected and stored at -80C until required ( 2.2 Cell viability assay Cell Counting Kit-8 (CCK-8) (Beyotime Biotech, China) was used to measure the corresponding optical density ratio marker cell viability levels in 96-well plates according to the manufacturers instructions

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SYBR-based quantitative PCR was conducted with the StepOne Plus Real-Time PCR System (Thermo Fisher Scientific) with specific primers for murine IL6 or HPRT (Thermo Fisher Scientific)

The resistance to MAPK, CDK, and ALK inhibitors also involves IGF-IR
Oral supplementation of glutamine attenuates the progression of nonalcoholic steatohepatitis in C57BL/6J mice
