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To test the effects of GHK and GHK-PEG on SH-SY5Yneuroblastomacell proliferation, cells were plated in 24 well plates and treated with GHK or PEGylated GHK at 10 and 20 nM for 24 hours and the effect of each treatment on cell proliferation was assessed using a hemocytometer

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Another epigenetic regulator of interest is JMJD3 (also known as KDM6B), a histone H3K27 demethylase that plays crucial roles in both vascular and cancer biology

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