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Definition, Uses, Effects Marijuana, derived from the Cannabis sativa plant, is one of the most widely used psychoactive substances in the U.S

DOI: 10.2217/epi-2023-0207 6

In de novo hypothyroid adults without cardiac disease, total daily doses of 20-25 mcg divided two or three times achieve physiologic free T3 levels for most patients

2.2 Laboratory-scale bioreactor cell culture At the laboratory scale, MRC-5 cells were cultured in 3 L glass bioreactors with a BioFlo 320 controller (Cat#: 1379962911, Eppendorf, Hamburg, Germany) on 1 g/L Cytodex 1 microcarriers (Cat#: 17548702, Cytiva, Marlborough, MA) or in iCELLis Nano fixed-bed bioreactors (Pall Corporation, Port Washington, NY) with a 10 cm bed height with WMEM and 10% BCS controlled at a target temperature of 37C and pH of 7.2 as described previously (Swartz et al., 2022)

Abbreviations ACC: Acetyl-CoA carboxylase CD36: Cluster of differentiation 36 CKD: Chronic kidney disease CoA: Acyl coenzyme A CPT1A: Carnitine palmitoyltransferase 1 DN: Diabetic nephropathy ECM: Extracellular matrix EMT: Epithelialmesenchymal transition ESRD: End-stage renal disease ESRRA: Estrogen-related receptor alpha FAO: Fatty acid oxidation FAS: Fatty acid synthase MF: Myofibroblasts PGC-1: Peroxisome proliferator-activated receptor coactivator 1 PPAR: Peroxisome proliferator-activated receptor RF: Renal fibrosis SCFAs: Short-chain fatty acids SIRT1: Sirtuin 1 STAT6: Signal transducer and activator of transcription 6 TCM: Traditional Chinese medicine TWIST1: Twist-related protein 1 References Djudjaj S, Boor P