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Pelleted cells were flicked and 400 L of a saponin-based permeabilization and wash reagent (0.2% saponin containing 4% FBS, 1% BSA and 0.02% Sodium Azide in PBS) was added and incubated for 15 min
Iron overload in human disease
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To choose the optimal dose of GSH for the in vitro experiment, we evaluated the efficacy of GSH at various doses from 1 to 5 mM (Supplementary Fig
2 (1), 134137 (2012) Shareef M, Ashraf MA, Sarfraz (2016) Natural cures for breast cancer treatment
