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No published data establishes an optimal rest period
The brain relies heavily on vitamin B12. That is because vitamin B12 plays an essential role in producing hormones, the chemical signals that your body sends to communicate with itself

International Journal of Toxicology (2014) 11

ARA290, a peptide derived from the tertiary structure of erythropoietin, produces long-term relief of neuropathic pain: An experimental study in rats and beta-common receptor knockout mice
Its about controlling every variable you can, because in science, precision isn't just a goal