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Deglycosylation of the NTD 0.55 mg/ml of enzyme and 1 mg/ml of the purified NTD were incubated on a rotating table at 4C with one of five deglycosylases: PNGase F (hydrolyzes the bond between asparagine and the first N -acetylglucosamine), Endo H (hydrolyzes the glycosidic linkage between the two core N -acetylglucosamines), Endo F1 (cleaves hybrid but not complex oligosaccharides), Endo F2 (leaves one N -acetylglucosamine attached to asparagine), and Endo F3 (does not cleave high mannose or hybrid glycan chains) (66, 67)