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Thus, these tests were capable to determine the antidepressant effects of drug

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These results are backed up by the finding of a strong increase of the concentration of Pycnogenol s metabolites and components in the synovial fluid, surrounding articular cartilage in the joints in osteoarthritis patients ( intake in osteoarthritis patients reduced gene expression of key catabolic and inflammation markers like MMP-3, MMP-13 and IL-1 by more than 50% within knee cartilage ( contributes to restoring health in damaged joints

The extracts were centrifuged at 10,000 rpm for 10 min at 4 C, and the supernatant was collected and used immediately for the assay at a dilution of 1:10 in PBS/EDTA