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For lysis, we used nitrogen cavitation, a method that disrupts the plasma membrane but preserves intracellular organelles and minimizes lysosomal protease release 71

After thawing, the cell lysates were heated and cooled down to RT to solubilize all triglycerides

Moreover, cardiac insufficiency is accompanied by cardiac enlargement, increased ventricular volume, and heart mass (125)

Rosenfeld RJ, Goodsell DS, Musah RA, Morris GM, Goodin DB, Olson AJ (2003) Automated docking of ligands to an artificial active site: aumenting crystallographic analysis with computer modeling

However, always tell us what medications you're currently taking so we can make sure there are no interactions