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The gRNAs were expressed using an U6gRNA-Cas9-2A-RFP (Sigma-Aldrich), or pSpCas9 BB-2A-Puro (PX459) v2.0 vector after transient transfection using Lipofectamine LTX following the manufacturers protocol
Age-related absorption issues : As people age, the ability to absorb B12 from food decreases
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