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Nat Rev Drug Discov:124 Shin JW et al (2020) Curcumin induces stabilization of Nrf2 protein through Keap1 cysteine modification

Tissue sections were imaged under a microscope (DMi8, Leica Microsystems, Wetzlar, Germany, with a Hamamatsu Orca Flash 4 v3 sCMOS camera) where fluorescence was excited with a lumencor spectra X using 550 nm (secondary antibody Alexa Fluor 594), 470 nm (secondary antibody Alexa Fluor 488) or 385 nm (DAPI), and exposure time was held constant for each color channel regarding magnification and staining

There is growing evidence that oxidative and non-oxidative modifications, including deamidation [129,130], as well as amino acid mutations associated with inheritable, early-onset forms of cataract [131], act synergistically to predispose crystallins to aggregation and cataract formation
