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Thereafter, the cells were lysed in TBS with 1% Triton X-100 and protease inhibitor cocktail for 30 min on ice

doi: 10.21037/tcr.2016.09.16

These data suggest that Dmrta2 functions as a transcriptional repressor through the recruitment of the NuRD complex

Keep current-use vials refrigerated while maintaining longer-term inventory frozen

(2015), it was shown that excessive mitochondrial fission induced by Drp1 activation leads to increased production of mitochondrial ROS, which activates the NLRP3 inflammasome, promoting inflammation (Park et al., 2015)
