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Schapira AHV, Cooper JM, Dexter D, Clark JB, Jenner P, Marsden CD

Furthermore, heightened levels of ROS and reactive nitrogen species generated by mitochondria can initiate oxidative and nitrosative stress, exacerbating damage to both mitochondria and cardiomyocytes (39)

Among these stages, hit identification and hit-to-lead optimisation represent critical and resource-intensive bottlenecks, as they define both the chemical viability of candidate molecules and their potential for downstream development 14

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The procedure includes the mixing of 0.5 ml from each glycated BSA sample with 0.25 ml of a 0.01% (w/v) solution of TNBSA before incubating for two hours at 37 C