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Principal component analysis (PCA) was conducted using MATLAB R2015a (The MathWorks, Natick, MA, USA) with the PLS Toolbox version 8.1 (Eigenvector Research, Inc., Manson, WA, USA)

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Both GHRH analogs and ghrelin mimetics were previously employed in our laboratory studies to evaluate pulsatile secretion of a particular hormone and its receptor interaction, and used together they helped to explore mechanisms related to them more broadly than did use of any single compounds in the research that were found within a database in PubMed

Unconjugated bilirubin and haemin (both Frontier Scientific Europe, Carnforth, Lancashire, UK) served as external standards

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