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DNA fragment encoding wild-type HMGB1, Flag-tagged GSTP, Flag-tagged GSTM, Flag-tagged HMGB1 were produced by high-fidelity PCR, and then cloned into pcDNA3.1 or pET28a vector

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Generation of water-ice crystals at a containers periphery (where heat transfer is greatest) can produce a salting out effect, whereby the protein and excipients become increasingly concentrated at the slower-freezing center of a container (21)

Similar content being viewed by others Introduction Necrotizing enterocolitis (NEC) is a leading cause of morbidity and mortality amongst premature infants and is characterized by intestinal mucosal inflammation and necrosis, leading to overwhelming sepsis 1