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Description
Growth medium contained StemSpan SFEM (Stem Cell Technologies, 09600) supplemented with 10% ES-Cult FBS (Stem Cell Technologies 06952), 1% penicillinstreptomycin (Invitrogen, 15140122), IL-3 (20 ng ml 1 ), IL-6 (20 ng ml 1 ), TPO (50 ng ml 1 ), Flt3L (50 ng ml 1 ) and SCF (100 ng ml 1 ) (PeproTech, 213-13, 216-16, 250-31L 315-14 and 250-03)
Treatment often focuses on: Methionine SAMe (when oxidative stress is controlled) Zinc and magnesium B6/P5P Avoiding folate-heavy protocols that worsen symptoms in this biotype 2
Its important to work with a qualified provider to determine the appropriate dosage and avoid potential risks associated with overuse
Vargas-Parra et al., obtained the same results
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