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Percutaneous lumbar laser discectomy involves using laser energy to vaporize a small volume of the NP resulting in reduced pressure

Briefly, total RNA from cells was isolated using a Pure link RNA Mini kit (Life Technologies, USA), according to the manufacturers protocols

Abbreviations ACAN: Aggrecan ADMSCs: Adipose mesenchymal stem cells AF: Annulus fibrosus AFSCs: Annulus fibrosus stem cells BMP: Bone morphogenetic protein BMSCs: Bone marrow mesenchymal stem cells CEPs: Cartilaginous endplates CESCs: Cartilage endplate stem cells COL I: Collagen type I CPCs: Chondrogenic progenitor cells DDDs: Degenerative disc diseases DHI: Disc height index ECM: Extracellular matrix EPO: Erythropoietin ESCs: Embryonic stem cells Exos: Exsomes GDF: Growth differentiation factor GDF-5-hIPSCs: GDF-5-transfected human IPSCs HA: Hyaluronic acid hTIMP: Metalloproteinase tissue inhibitor IDD: Intervertebral disc degeneration IFN: Interferon IL: Interleukin IPSCs: Induced pluripotent stem cells IVD: Intervertebral disc IVDSCs: Intervertebral-derived stem cells MAPK: Mitogen activated protein kinase MMPs: Matrix metalloproteinases MSCs: Mesenchymal stem cells MSCs-SEVs: Derived small extracellular vesicles NCs: Notochord cells NP: Nucleus pulposus NPSCs: Nucleus pulposus stem cells PGE: Prostaglandin E PSCs: Pluripotent stem cells SAG: Smoothened agonist Shh: Sonic hedgehog TBT: Transgenic BMSCs TEC: Tissue engineering structure TNF: Tumor necrosis factor UCMSCs: Umbilical cord mesenchymal stem cells WJMSCs: Wharton's Jelly mesenchymal stem cells References Batti MC, Joshi AB, Gibbons LE