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Another example of PMA-activated NF-B activation can be found in the human mammary epithelial cells, where sulforaphane inhibits both IKK activation and ERK1/2i.e., ERK1/2-IKK-NF-B signalling [140]

Transverse 10 m sections were obtained on a rotary microtome (Leica RM2255), deparaffinised, and stained with Massons trichrome according to Servicebios protocol (Wuhan, China)

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