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Raw sequencing data was demultiplexed with bcl2fastq2 v.2.19.0.316 (Illumina) with adapter trimming turned off

USA 108, E1184E1191

As usual, three knots were adopted in the study according to a previous study and the suggestion by Harrell ( p -values less than 0.05 were considered as statistically significant for all these analyses

Hu CW, Wu MT, Chao MR, Pan CH, Wang CJ, Swenberg JA, Wu KY: Comparison of analyses of urinary 8-hydroxy-2-deoxyguanosine by isotope-dilution liquid chromatography with electrospray tandem mass spectrometry and by enzyme-linked immunosorbent assay

Hematoxylineosin (H&E) staining and immunohistochemistry The submandibular gland tissues embedded in paraffin were cut into 4-m-thick slices and then stained with hematoxylin and eosin (H&E) to evaluate inflammatory cell infiltration and histopathological damage