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However, its common for blood tests to reveal inaccurate information regarding B12 levels since a high percentage is stored in the liver and not always revealed through testing (for example, taking large amounts of folic acid has been found to mask vitamin B12 deficiency in some patients)

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Whether the His tag is exposed on the surface of the fusion protein can be determined by adding an appropriate amount of denaturants such as urea or guanidine hydrochloride to the protein
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