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In the next step, samples were diluted with 100 mM ABC, then reduced with 5 mM TCEP (in 50 mM ABC) for 30 min at 37 C, re-alkylated with 10 mM d5-NEM, diluted with four volumes of 25 mM ABC and digested overnight with trypsin at 37 C, (shaking at 550 rpm
The interest of researchers should be directed into the development of new serological parameters useful in the monitoring of the disease, as well as verifying the efficacy of ongoing treatment
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10.3389/fimmu.2022.934695 102 RosasH
[6] Frias JP, et al
