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Isolation Necrotic tissue from a wound or wound exudate can be heated to 80C for 20 minutes and used to inoculate a blood agar plate and another blood agar plate containing 3% agar (stiff agar)

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Exp Physiol 108:1227 [DOI] [PMC free article] [PubMed] [Google Scholar] 7.Badenoch JB, Rengasamy ER, Watson C, Jansen K, Chakraborty S, Sundaram RD, Hafeez D, Burchill E, Saini A, Thomas L, Cross B, Hunt CK, Conti I, Ralovska S, Hussain Z, Butler M, Pollak TA, Koychev I, Michael BD, Holling H, Nicholson TR, Rogers JP, Rooney AG (2022) Persistent neuropsychiatric symptoms after Covid-19: a systematic review and meta-analysis

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