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Dihexa (PNB-0408) is an angiotensin IVderived peptide studied in preclinical models for its role in synaptogenesis and cognitive signaling mechanisms

Prior to any procedures, animals were anesthetized using a combination of xylazine (10 mg/kg), ketamine (40 mg/kg) and acepromazine (0.75 mg/kg) for up to one hour via the intramuscular route

Quantification was done by integration of the extracted ion chromatogram peaks for the following ion species: m/z 45 for acetate eluted at 7.8 min, m/z 74 for propionate eluted at 9.6 min, and m/z 60 for butyrate eluted at 11.5 min

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