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Neural Regeneration Research, 17 (3), 482-487

Distal colon content (200 mg) from two mice for one experiment was homogenized in PBS (1 mL) and centrifuged at 9000 g , the supernatant was collected, and then the low-molecular-weight fraction of the supernatant was fractionated using Amicon Ultra (Ultracel 3 K, Merck, Darmstadt, Germany) and the high-molecular-weight fraction was fractionated using a PD SpinTrap G-25 column (GE Healthcare, Buckinghamshire, UK)

Institutional review board statement The study was conducted following the Good Clinical Practice standards and the Helsinki Declaration, and approved by the Ethical Committee of the University of Cagliari (protocol PG/2018/16313)

Each box contains 30 separately packed sheet masks, designed for convenient, hygienic use and consistent daily care

Packaging is handled with discretion and care to maintain product integrity during transit