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The gradient profile was: 3% solvent B for 5 min, increased to 20% over 4 min, further increased to 50% over 2 min, then to 95% over 1 min, followed by re-equilibration to 3% for 2 min (total run time 14 min)

We found virtually no group differences for changes from baseline in metabolite or pH levels (ie, significant treatment-by-time interactions in our model), including a posteriori analysis of changes in total NTP, at week 1 or week 12 (Table 3)

Thus, high doses of caffeine are regarded to increase renal clearance and promote more rapid excretion of many drugs and their metabolites from the body

Autophagy in kidney homeostasis and disease

PubMed, Sturza, Adrian, et al