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Pennsylvania Student Sustainability Symposium Location: Dickinson College Jill Marie Hautaniemi Changes in tree diameter from the edge to the interior of Buger community forest reserve in Tanzania Date: March 21, 2014 Event: 2014 Pennsylvania Environmental Resource Consortium

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Plasmids and gRNA library construction We modified CROPseq-guide-Puro (Addgene plasmid #86708) lentiviral plasmid 29 to embed the gRNA sequences in the 3 UTR of a poly-adenylated RFP transcript, generating the CROP-seq-RFP lentiviral vector