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Co-immunoprecipitation mass spectrometry and CRISPR KO in cells identified that mitochondrial m -AAA protease AFG3L2 is responsible for degrading SLC25A39 through the matrix loop 1

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In vitro studies confirm minimal cytotoxicity with no impact on cell viability up to 100 M concentrations across multiple cell types, though higher concentrations may produce non-specific effects that could confound experimental results

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