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ACS Mater

Phase IIa, placebo-controlled, randomised study of lutikizumab, an anti-interleukin-1 and anti-interleukin-1 dual variable domain immunoglobulin, in patients with erosive hand osteoarthritis

Khan, R

Material and methods Cell culture Human hepatocellular carcinoma (HepG2) (ATCC, HB-8065) were maintained in DMEM (4.5 g/l glucose, pyruvate) (Gibco), supplemented with 10% fetal bovine serum (PAA Laboratories) and penicillin/streptomycin (25 g/ml each, Gibco) at 37 C, 5% CO 2

Following a 30-minute equilibration period, active vessel tone was verified by assessing arteriolar dilation in response to 100 M adenosine