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Description
43,44 In 1984, Krieg and Melton used RNA polymerase extracted from viruses for in vitro transcription from engineered DNA templates, successfully achieving mRNA expression in cell-free systems
PMID: 32217857
After amplification, the double-stranded DNA product was purified using a Monarch PCR & DNA Cleanup Kit (New England Biolabs)
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21:E8765
