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Next, we investigated whether LIP-expressing cells depend on the increased glycolytic flux for cell proliferation and survival

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RNA from microdissection samples were extracted in 300 L TRI-Reagent, precipitated in 500 L isopropanol overnight at 20 C, solubilized in 20 L of RNase-free water and quality checked using Total RNA 6000 Pico chips and the 2100 Bioanalyzer

Proc Natl Acad Sci India Sect B Biol Sci

Fertility and Sterility