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A 10mM erastin2 stock solution was prepared by dissolving it in dimethylsulfoxide

Comparison and evaluation of two different methods to establish the cigarette smoke exposure mouse model of COPD

Patch pipettes (24M) were pulled from borosilicate glass (1.6 mm outside diameter) and filled with intracellular solution containing (in mmol/L): 130 HMeSO 4 , 5 KCl, 1 EGTA, 10 HEPES, 4 MgCl 2 , 2 ATP-Na 2 , 0.4 GTP-Na, 7 Phosphocreatine-Na 2 , with pH adjusted to 7.27.4 by KOH, and osmolarity adjusted to 290295 mOsm

Downstream Signaling Pathways Following target engagement, several downstream signaling cascades have been reported in experimental models

Science 262, 17441747