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Cell culture and stimulation Normal human epidermal keratinocytes (NHEKs) were purchased from Takara Bio (Shiga, Japan) and cultured in Keratinocyte Basal Medium (KBM) overnight prior to human recombinant rIL-22 stimulation (200 ng ml 1 , R&D Systems, Minneapolis, MN)

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Results of the study showed glutathione levels in the blood increased after one, three and six months vs

Some of them can be prevented, while others cannot