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Initially, trimethyllysine undergoes hydroxylation by trimethyllysine dioxygenase (TMLD), resulting in the formation of hydroxytrimethyllysine
The researchers examined the impact of these interventions through a biochemical characterization of brain homogenates, quantifying oxidative and inflammatory markers such as lipid peroxides, glutathione (GSH), glutathione S-transferase (GST), 5-lipoxygenase (5-LOX), cyclooxygenase-2 (COX-2), leukotriene B4, prostaglandin E2, and ascorbic acid using spectrophotometric assays and ELISA

To determine whether GPI-MFI2 co-localizes with caveolae or clathrin-coated pits, we employed a modified internalization assay previously used in measuring the endocytosis of other membrane receptors [49]

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