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Footnotes CONFLICT OF INTEREST The authors declare the following potential conflicts of interest with respect to the research, authorship, and/or publication of this article: Yeast extract (YE) was obtained from Samyang Corporation

An adequate amount of soap is required to dissolve fatty materials and oils from hands as water alone is not sufficient to clean soiled hands

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Oncolytics 16 , 124133 (2020)

Abbreviations BBD: Box-Behnken design BSA: Bovine serum albumin DTNB: (5,5-dithiobis-(2-nitrobenzoic acid) GPx: Glutathione peroxidase GSH: Reduced glutathione GSSG: Oxidized glutathione LOD: Limits of detection LOQ: Limits of quantitation RSM: Response surface modeling TBuOOH: Tert-Butyl hydroperoxide TNB: Nitro-2-thiobenzoate anion WSAVA: World Small Animal Veterinary Associations References Ademi M (2023) The role of ionized water and its impact on the activity of glutathione peroxidase on rats blood serum during hyperthermic stress