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When cultured in YP20D medium supplemented with 14 mM acrolein, the parental KACC 48331 strain failed to grow, whereas 21 out of 376 UV-induced mutants exhibited detectable growth

Following a 30-minute incubation at 37C in the dark, the cells were washed three times with 0.01 M PBS

All experiments involving PSC were performed between passages 3 and 10

Organ dysfunction has recently been reported to be closely related to coronavirus disease (COVID-19) progression, which may cause acute kidney injury (AKI)

Also, the study has some limitations, such as the fact that the researchers only used aged female mice for their experiments