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In addition, intracellular scaffold proteins interface with the actin cytoskeleton, linking transmembrane tight junction proteins to the cytoskeleton and subsequently acting as a molecular target for tight junction dysregulation in various pathways, including the Zot and zonulin pathways, which both involve the dysregulation of tight junction proteins to induce alterations in intestinal permeability (7, 1115)

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For fixing the biological material, brains and organs were fixed in paraformaldehyde (4%) at pH 7.4 for overnight and dehydrated in sucrose gradient (1530%) followed by cryosectioning

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In other major organs including the lung, heart, and spleen, the concentration was approximately 110 fold within 15 min (0.25 h)