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In short, genomic DNA was extracted by cell lysis (10 mM Tris-HCl, 10 mM EDTA, 150 mM NaCl and 0.1 % SDS), proteinase K treatment (New England Biolabs) and DNAse-free RNAse digest (Thermo Fisher), followed by two rounds of phenol extraction and isopropanol precipitation
The authors concluded that while the combination showed limited effectiveness in the overall population, it might offer potential benefits for individuals with particular baseline features [76]

1985 May;17(5):48593

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Emerging pathogenic links between microbiota and the gut-lung axis