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The supernatant was filtered through a 0.22 m filter to remove residual particles, with a portion of the filtrate transferred to a detection vial, where 20 L was used to prepare quality control (QC) samples, and the remainder was utilized for liquid chromatography-mass spectrometry (LC-MS) analysis

This suggests that the metabolite is the primary effector of the detrimental effects of heroin on the BBB

[DOI] [PMC free article] [PubMed] [Google Scholar] 225.Hausmann M., Obermeier F., Paper D., Balan K., Dunger N., Menzel K., Falk W., Schoelmerich J., Herfarth H., Rogler G

NU9056, a KAT 5 inhibitor, treatment alleviates brain dysfunction by inhibiting NLRP3 inflammasome activation, affecting gut microbiota, and derived metabolites in LPS-treated mice