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Furthermore, studies have shown that alcohol withdrawal is associated with increased excitatory amino acid transmission, leading to manifestation of symptoms such as seizures, which can be blocked using NMDA receptor antagonists (Danysz et al., 1992

Briefly, Kokas extensor postural thrust (EPT) i.e., modified motor function index (MFI) assessed by an experienced, but naive, co-tester, as the weight on a digital weighing scale (precision 0.001 g) borne by the hind limb at the moment when the rat was held upright, with the hind limb extended and placed upon a scale, begins to bear weight on the scale, as follows: EPT = (NEPTEEPT)/NEPT (NEPT and EEPT represent EPT value before surgery (normal) and after surgery (experimental), respectively)

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The brains of the mice were removed, postfixed with the same fixative for 24 h at 4C, and then cryoprotected using a graded series of buffered sucrose solutions (20% and 30%)